In deprived of triose phosphate isomerase, only one of the two three-carbon molecules produced by aldolase might be used to produce ATP in which only two molecules of ATP would outcome from the metabolism of each molecule to glucose but two molecules of ATP would still be essential to form fructose 1,6-biphosphate, the sub state for aldolase. The net yield of ATP would be 0, a yield incompatible with life.
