Respuesta :
The Primer is a short DNA fragment to synthesize the new DNA strands.
The target sequence's flanking region's sequence is complementary to the primers' brief synthetic single-stranded DNA molecules. Any desired base sequence for primers can be produced chemically through the chemical synthesis of these DNA molecules. Primers are over the template by millions of folds since each new DNA strand will start with one. Thus, it is essential to understand the template sequence on each side of the DNA region that needs to be amplified. Biotechnology supply businesses offer commercial DNA polymerases and dNTPs. By extending DNA from the primer's 3' end, the DNA polymerase duplicates DNA and creates a new DNA strand. Similar to cellular replication, there are twice as many DNA molecules at the end of the first cycle. However, with PCR, steps 1-3 are performed repeatedly, typically for 25 to 30 cycles.
Hence, primer is short sequence provided in PCR to initiate polymerization.
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