The covalent phosphodiester bonds of DNA are hydrolyzed by restriction enzymes, leaving either "sticky/cohesive" ends or "blunt" ends.
By incubating the target DNA molecule with restriction enzymes, which detect and bind certain DNA sequences and cleave at specified nucleotides either inside or outside of the recognition sequence, restriction digestion is carried out.
An isolated bacterial protein known as a restriction enzyme cleaves DNA at sequence-specific locations to create DNA fragments with a known sequence at either end. Restrictions enzymes are crucial for numerous laboratory processes, including recombinant DNA technology and genetic engineering.
At the particular restriction site, DNA bonds between the 3′ OH of one nucleotide and the 5′ phosphate of the following one are cleaved by restriction enzymes.
In order to prevent the plasmid vector from ligating with itself and to verify that the inserted gene is oriented correctly, two separate restriction enzyme sites might be used.
A) #1 5′ - CGTGATCTCGATTCGCTAGTAACGTT - 3′
3′ - GCACTAGAGCTAAGCGATCATTGCAA - 5′
#2 5′ - TCATGAATTCCTGGAATCAGCAAATGCA - 3′
3′ - AGTACTTAAGGACCTTAGTCGTTTACGT - 5′
B) Recognition sites:
#1 5′ - GAATTC - 3′
3′ - CTTAAG - 5′
#2 5′ - GAATTC - 3′
3′ - CTTAAG - 5′
C) Cleavage sites:
#1 5′ - G AATTC - 3′
3′ - CTTAA G - 5′
#2 5′ - G AATTC - 3′
3′ - CTTAA G - 5′
The correct and complete question is in the image.
Learn more about Restriction enzymes here:
https://brainly.com/question/13944056
#SPJ4
