Answer:
Separation 2 has the highest resolution value which is 3, it is therefore the most desirable.
Explanation:
Note that in the given separations, the values for compound B which are missing in separation 3 were erroneously put in separation 4. These are the correct values for separations:
a. Separation 1: compound A: t; = 13.0 min; w = 5.0 min compound B: t; = 17.0 min; w = 6.5 min
b. Separation 3: compound A: tr = 14.0 min; w = 4.0 min compound B: tr = 16.0 min; w = 5.0 min
c. Separation 2: compound A: t; = 10.0 min; w = 3.0 min compound B: t; = 19.0 min; w = 3.0 min
d. Separation 4: compound A: tr = 11.0 min; w = 2.0 min compound B: tr = 15.0 min; w = 3.0 min
e. Separation 5: compound A: tr = 9.0 min; w = 2.5 min compound B: t; = 13.0 min; w= 4.5 min
Determination of chromatographic separation that achieved a resolution that is desirable for quantitative analysis
In chromatography, the formula for Resolution (R) = 2(tR(B) - tR(A)) / (WB + W A)
For separation 1 : Resolution (R) = 2(17.0 min - 13.0 min) / (6.5min + 5.0min ) = 0.695
For separation 2 : Resolution (R) = 2(19.0 min - 10.0 min) / (3.0min + 3.0min ) = 3
For separation 3 : Resolution (R) = 2(16.0 min - 14.0 min) / (5.0min + 4.0min ) = 0.444
For separation 4 : Resolution (R) = 2(15.0 min - 11.0 min) / (3.0min + 2.0min )
= 1.6
For separation 5 : Resolution (R) = 2(13.0 min - 9.0 min) / (4.5min + 2.5min )
= 1.14
A chromatographic separation with a higher resolution is desirable for quantitative analysis. Since separation 2 has the highest resolution value which is 3, it is therefore the most desirable.
