Affinity chromatography is used to purify a protein of interest by capturing it on Select one:

a. matrix material with a strongly interacting molecule (very high dissociation constant with target protein) attached.
b. matrix with very small pores that will only allow the protein of interest to pass.
c. matrix material with a strongly interacting molecule (very low dissociation constant with target protein) attached.
d. matrix material with molecules of opposite charge attached (relying on attraction between positive and negative charges).

Affinity chromatography (AC) is based on the immobilization of the protein of interest inside of the column, by means of having a strongly interacting molecule in the matrix.

Strongly interacting means that the complex Protein-Interacting Molecule will not be easily dissociated, this translates into a low dissociation constant.

ayfat23 ayfat23 · Answer 2 · 2022-01-07T16:43:57+01:00

It should be noted that Affinity chromatography is used to purify a protein of interest by capturing it on matrix material with a strongly interacting molecule (very low dissociation constant with target protein) attached.

Affinity chromatography can be regarded as a way of separating a biomolecule from a mixture, this is been done with regards to how high the specific macromolecular binding interaction that exist between the biomolecule as well as the substance involved

Therefore, chromatography is used to purify a protein of interest by capturing it on matrix material.

Learn more about chromatography at:

https://brainly.com/question/15004409