In the lab, your tlc sheet is done and you have heated it with the hair dryer. If you have free amino groups (in amino acids) on your tlc sheet, the ____________ (chemical substance) in the solvent will turn orange, pink, purple or violet.

Respuesta :

The given blank can be filled with ninhydrin.  

It refers to a synthetic crystalline component, which produces deeply colored products with primary amines and is utilized in analytical tests for amino acids. It is a chemical that is generally utilized to determine primary or secondary amines.  

In the ninhydrin test, the amines react with ninhydrin to produce a colored component. It can be used quantitatively (for peptide sequencing) or qualitatively (for chromatographic visualization). The alpha-amino acids usually give a blue-purple product.  


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