The food web above represents feeding relationships in a biological community near a deep-sea hydrothermal vent. Hydrothermal vents are geysers on the seafloor that gush super-heated, mineral-rich water. The seawater surrounding hydrothermal vents typically contains carbon dioxide (CO2), molecular hydrogen (H2), hydrogen sulfide (H2S), and methane (CH4). Sunlight, however, fails to reach the seafloor where deep-sea hydrothermal vents are located.
As part of an investigation, researchers collected living specimens from an area near a deep-sea hydrothermal vent. Mussels in the collection were found to be dependent on molecular hydrogen in seawater. Also, the researchers discovered multiple species of bacteria living in the gills of the mussels. Mussels use gills for filter-feeding and gas exchange with the surrounding seawater. On the basis of their experimental results, the researchers hypothesized that some bacteria living in the gills of the mussels are capable of chemosynthesis. Researchers are investigating the evolutionary relationships among organisms found near deep- sea hydrothermal vents and similar organisms found closer to the ocean surface. Which of the following scientific questions is most relevant to the investigation?